Abstract:ObjectiveTo study the ZTC1+1 clarification procedure of Yishentongluo granule. Methods ZTC1+1 Ⅱ nature clarifying agent was applied to the clarification process of Yishentongluo granule. The optimum clarifying temperature, the density of decoction and the content of ZTC1+1 nature clarifying agent were evaluated according to the content of salvianolic acid B、total polysaccharidet and the yield of dried extract and its effect were compared with alcohol sedimentation. ResultsBesides reducing the yield of dried extract, the ZTC1+1 was more effective than alcohol sedimentation in retaining the active compound. ConclusionThe ZTC1+1 Ⅱ nature clarifying agent used in the clarification process of Yishentongluo granule is feasible.
Key words:Yishentongluo Granule; ZTC1+1 nature clarifying agent; Clarification process
2.4.1 絮凝温度的考察量取原药液100 ml 3份,分别将药液加热至60,70,80℃,保温,搅拌分散加入3 ml 1%B组分,之后每隔30 min搅拌`1次,2 h后,将药液温度降至60℃,同法加入1.5 ml 1%的A组分,将药液温度升至80℃,保温30 min即可。静置4 h,测定。结果见表1。70℃时综合评分最高,故絮凝的最佳温度选择70℃。表1 絮凝温度考察结果(略)
综合评分=丹酚酸B保留率×100×0.4+多糖保留率×100×0.4+固形物减少率×100×0.2
2.4.2 药液浓缩比的考察量取原药液100 ml 5份,分别稀释至生药体积比(g/ml)为1∶5,1∶7.5,1∶10,1∶15,1∶20,将药液温度加热至70℃,保温,加入3 ml B组分,其它操作同“絮凝温度的考察项下”。结果见表2。生药体积比为1∶7.5时综合评分最高。表2 药液浓缩比考察结果(略)
2.4.3 澄清剂用量的考察量取原药液100 ml 3份,分别稀释至生药体积比为1∶7.5,将药液温度加热至70℃,保温,B组分的加入量分别为2%,3%,4%药液体积,A组分的加入量为相应B组分的一半,具体操作同“絮凝温度的考察项下”。结果见表3。加入2%B组分时综合评分最高。表3 澄清剂用量考察结果(略)
2.4.4 醇沉与澄清剂工艺比较量取药液100 ml 3份,加入乙醇,使含醇量分别达到30%,50%,70%,静置过夜,滤过,滤液浓缩至100 ml,分别测定各指标。结果见表4。
取相同药液100 ml 3份,分别稀释至生药体积比为1∶7.5,搅拌分散加入2 ml B组分,之后每隔30 min搅拌1次,2 h后,将药液温度降至60℃,同法加入1 ml的A组分,将药液温度升至80℃,保温30 min即可,静置24 h,依照2.3项下方法测定,结果见表4。表4 醇沉与澄清剂工艺比较(略)