Abstract:ObjectiveTo establish an HPLC method for the determination of Carbamazepine in Compound Kagai Changpu Capsules.MethodsAn HPLC assay was developed. Chromatographic assay was performed on a column of Hypersil C18(200 mm×4.6 mm,5 μm),using methanol-0.02 mol/L KH2PO4(60∶40)as the mobile phase with the flow rate of 1.0 ml/min.The detection wavelength was at 285 nm.20 μl sample and external method were used.ResultsA satisfactory separation was obtained.The calibration curve was linear over the range of 0.008 648~ 0.172 96 μg for ribavirin (r=1.000 0,n=9).The average recovery of ribavirin was 101.44%(n=9) with RSD of 0.49%.ConclusionThe method is quick and simple with good reproducibility.It can be used for the quality control of Compound Kagai Changpu Capsules.