Abstract:ObjectiveTo establish an HPLC method for determination of Caffeic acid in Xiaoyan Tuire Granule.MethodsThe separation was carried out. ODS column(AIltima,5 μm,4.6 mm×250 mm) was used mobile phase of Acetonitrile- 0.4% Phosphoric acid (13∶87) and detection wavelength of 323 nm.ResultsThe Caffeic acid samples showed a good linear relationship at the range of 0.0387~0.387 0 μg(r=0.9998,n=6). The average recovery of the added sample was 100.56%(n=6) and method precision was RSD=1.36%(n=6).ConclusionThe method is simple, available and accurate