Extraction Study of Berberine from Cortex Phellodendri by Ultrasonic Wave- Cellulase
XU Yan,LIU Shaoxia,SUN Juan
School of Bioscience and Technology,Shenyang Agricultural University,Shenyang 110161, China
Abstract:ObjectiveTo study the application effect on extraction of berberine from Cortex phellodendri by ultrasonic wave- cellulase.MethodsBased on the single factors test and orthogonal test the effect of different factors on extraction of berberine from Cortex phellodendri by ultrasonic wave-cellulase was studied,and extracting conditions was optimized.ResultsThe best extraction condition was that time was 2.5 h, the power of ultrasonic wave was 80 ℃ , the volume of cellulase was 25 ml, temperature was 60 ℃.ConclusionThe berberine of Cortex Phellodendri is extracted by Ultrasonic wave-Cellulase ,which can save time and enhance extract-efficiency, save organic solvent and reduce the use of energy .