Abstract:ObjectiveTo establish HPLC method for determination of Gastrodin in Tianma Granule.MethodsThe analytical column was a nucleosil C18 (10 μm ,4.6 mm×250 mm).The mobile phase consisted of acetonitrilewater(3∶97).The flow rate was 1.0 ml·min-1 and the column temperature was 25℃ .The determination wavelength was 220 nm . ResultsThe calibration curve was linear in the range of 10.83~64.98μg·ml-1 (r=0.999 9).The regression equation was A=99.1571C-21.266 7.The average recovery was 99.2%,RSD was 0.6%(n=5). ConclusionThe method is simple , reliable and reproducible for quality control of Gastrodin in Tianma Granule.