Determination of Ginsenoside Rg1 in Wulongdan by HPLC
Abstract:Objective To estabish a method for determination of ginsenoside Rg1 in Wulongdan. MethodsGinsenoside Rg1 was determined by HPLC.The mobile phase was acetonitrile- 0.2 moL/L phosphoric acid (83∶17) at a flow of 1.0 ml/min, and detection wavelength was 203nm. ResultsThe determination method for ginsenoside Rg1 had a good linear relationship in the range of 0.404~4.04 μg, r=0.999 6. The average recovery was 96.34%, RSD=2.37%. ConclusionThis method is simple, accurate and reliable, which can be used for determination of the contents of ginsenoside Rg1 in Wulongdan.