Study on Determination Methods of Total Flavonoids and Quality Identification about Eucalyptus youmanii Leaves
Abstract:ObjectiveTo study the determination methods of total flavonoids and quality identification about Eucalyptus youmanii leaves.MethodsThe content of total flavonoids in leaves of Eucalyptus youmanii which was induced to Sichuan was detected by UV spectrophotometric method, AlCl3 colorimetric method, Al(NO3)3 colorimetric method and HPLC. Comparisons of the four methods were also discussed. ResultsThere were two types with different quality and chemical components in which the A259/A364 of extracting liquid from leaves by methanol was 1.71 and the other one was 2.34. The determination results among the four methods in leaves (A259/A364≈1.71) had no obvious difference but the results by Al(NO3)3 colorimetric method in leaves (A259/A364≈2.34) was preponderate 46.4% and 39.9% over HPLC and AlCl3 colorimetric method. ConclusionThe trees (A259/A364≈2.34) are likely to hold some components disturbing Al(NO3)3 colorimtric method. AlCl3 colorimetric method is superior to UV spectrophotometric method and Al(NO3)3 colorimetric method. The two types are easy distinguished by method of extraction with hot water, UV spectrophotometric method and HPLC.
Key words:Eucalyptus Youmanii leaves; Total flavonoids; Determination method; Quality identification