Abstract:ObjectiveTo establish the determination method of flavonoids in compound Ginkgo biloba Leaves Tablets .MethodsThe analysis was performed on a SymmetryC18 column(4.6 mm ×250 mm,5 μm) with methanol 0.4% phosphoric acid( 52:48 ) as mobile phase at a flow rate of 1.0 ml ·min-1, and at a column temperature of 35℃. The detection wavelength was 360nm.ResultsThe linearity of this method was good.The average recovery of added samples was 98.35 % with RSD 1.17 %. (n =5 ) .ConclusionThe method is convient with a good separating degree for the determination of flavonoids and can be used to evaluate the quality of compound Ginkgo biloba Leaves Tablets.