Abstract:ObjectiveTo find out the differences of stachydrine content in Leonurus japonicus Houtt. from different regions and its different parts. MethodsField investigation and laboratory analysis were made on Leonurus japonicus Houtt. The stachydrine content was determined by TLC-scanning. ResultsThe calibration curve of stachydrine was linear over the range of 1~20μg. The regression equation was y=5 381x-7 724.6,r=0.999 2(n=6). The average recovery and RSD were 97.4% and 2.70%(n=8) respectively. ConclusionThere existed significant differences in the stachydrine content from different regions. Among them, Leonurus japonicus Houtt. from Beijing, Henan and Shandong are better. The differences of stachydrine content in the different part of Leonurus japonicus Houtt. is significant, and that in progenitive part is better than in leaf, which in the stem is the lest.
Key words:Leonurus japonicus Houtt.; Stachydrine; Different regions; Different parts