The Effect of Ginseng Polysugar on Secretion Function of Luteal and Granulose Cell of Rat in vitro
Abstract:ObjectiveTo study using of ginseng polysugar on secretion function of female rat ovary. The experiment had observed progesterone and cAMP by HCG inducing luteal and granulose cell as well as living cell rate of oocyte in vitro.MethodsRat luteal and granulose cell and oocyte was cultivated, cell counting was worked by microscope, progesterone and cAMP content was determined by radioimmunoassay kit(RIA kit). Cell exist rate of oocyte was determined by MTT way. ResultsProgesterone: the luteal cell test groups compare with control group, 6573.46±185.14 and(4509.55±126.82)pmol.The granulose cell test groups compare with control group, 197.16±42.87 and (320.42±12.46)pmol.cAMP: the luteal cell test groups compare with control group, 31.20±17.13and(65.26±15.93)fmol,the granulose cell test groups compare with control group, 121.15±19.96 and (296.42±27.28)fmol.ConclusionThis treatise had demonstrated that ginseng polysugar can inhibit HCG inducing progesterone secretion and enhancing cAMP content of luteal and granulose cell of rat, ginseng polysugar has lower oocytes cell grow up inhibitory rate. It is evident that ginseng polysugar possesses characteristic of dose dependent.
The Effect of Ginseng Polysugar on Secretion Function of Luteal and Granulose Cell of Rat in vitro
Abstract:ObjectiveTo study using of ginseng polysugar on secretion function of female rat ovary. The experiment had observed progesterone and cAMP by HCG inducing luteal and granulose cell as well as living cell rate of oocyte in vitro.MethodsRat luteal and granulose cell and oocyte was cultivated, cell counting was worked by microscope, progesterone and cAMP content was determined by radioimmunoassay kit(RIA kit). Cell exist rate of oocyte was determined by MTT way. ResultsProgesterone: the luteal cell test groups compare with control group, 6573.46±185.14 and(4509.55±126.82)pmol.The granulose cell test groups compare with control group, 197.16±42.87 and (320.42±12.46)pmol.cAMP: the luteal cell test groups compare with control group, 31.20±17.13and(65.26±15.93)fmol,the granulose cell test groups compare with control group, 121.15±19.96 and (296.42±27.28)fmol.ConclusionThis treatise had demonstrated that ginseng polysugar can inhibit HCG inducing progesterone secretion and enhancing cAMP content of luteal and granulose cell of rat, ginseng polysugar has lower oocytes cell grow up inhibitory rate. It is evident that ginseng polysugar possesses characteristic of dose dependent.
[2] Dorfman M, Arancibia S, Fiedler JL, et al. Chronic intermittent cold stress activates ovarian sympathetic nerves and modifies ovarian follicular development in the rat[J].Biol Reprod, 2003,68(6):2038.
[3] Sternberg WF, Chesler EJ, Wilson SG, et al. Acute progesterone can recruit sex-specific neurochemical mechanisms mediating swim stress-induced and kappa-opioid analgesia in mice[J]. Horm Behav,2004,46(4):467.
[4] Luza SM, Arancibia S, Venegas M, et al. Thyrotropin-releasing hormone as a mediator of the central autonomic pathway controlling ovarian function[J].Neuroendocrinology, 2003,77(4):273.
[5] Arancibia S, Estupina C, Pesco J, et al. Responsiveness to depolarization of hypothalamic neurons secreting somatostatin under stress and estrous cycle conditions: involvement of GABAergic and steroidal interactions[J]. J Neurosci Res, 1997,50(4):575.