Preparation of Caffeic Acid Tetramers Reference Substance from Arnebia euchroma
Abstract:ObjectiveTo prepare reference substance for quality control of Arnebia euchroma. Caffeic acid tetramers was extracted and separated from acetone water extraction of Arnebia echroma.MethodsChemical structure of the caffeic acid tetramers product was elucidated by ultraviolet spectrometry(UV),infrared spectroscopy(IR),nuclearmagnetic resonance(NMR) and mass spectrometry(MS). ResultsThe results were in agreement with the reference.The purity of the caffeic acid tetramers product was 98.11%. ConclusionThe method meets the reference substances of traditional Chinese medicines and can used to prepare and purify the active constituents from Arnebia euchroma.