Caco-2 Cell Model and Development of its Application to Absorption of Food Nutrients
Abstract:This article overviewed the characteristics of Caco-2 cell, establishment and validation of Caco-2 cell model. The research development of the mechanisms of absorption and transport of nutrients extensively was also reviewed. This model has many advantages, as well as few disadvantages.
Key words:Caco-2 cell; Nutrients; Absorption
对于营养物质在人体内的吸收转运及其生物利用度,不同的动物之间、动物与人之间都存在或大或小的质的差异。这种种属间的差异使得动物实验数据不能完全地外推至人体,并且直接利用人体组织进行研究是非常有限的。人类细胞培养系统作为肠屏障的体外模型的应用是模拟营养物质透过小肠黏膜的机制与影响因素的一个有效的方法。它可采用人体或与人体最接近的生物材料,从而消除了动物模型和人体的巨大差异;有利于探讨营养物质动力学-结构的定量构效关系的特点;有高通量、节省时间、节省资金、有利于对组合代谢产物进行分析的特点。近十几年来,国外已普遍采用组织细胞模型作为营养物质吸收研究的工具,包括有Caco-2细胞(the human colon adenocarcinoma cell lines)单层模型、MDCK细胞模型、MDR1-MDCK 细胞模型及ECV304细胞模型。其中尤其是Caco-2细胞模型以其与体内营养物质,特别是那些被动吸收的营养物质研究的良好相关性,被普遍用于营养物质开发的早期快速筛选过程中[1,2]。
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