Study on the Separation and Purification of Total Flavone from Herb Cirsii japonici by Macroporous Absorption Resin
Abstract:ObjectiveTo select the suitable resin and establish the optimal conditions for separation and purification of total flavone from herb Cirsii japonici. MethodsTo determine the optimal one by inspection of four types of macroporous resin. The optimal process of purification was studied by single factor analysis .ResultsThe static absorption capacity, the absorption ratio and the static elution ratio of AB-8 resin was higher than the others . The optimal technological conditions were found in AB-8 as follows:sample concentration was 5.2 mg/ml,flow rate was 0.5 ml/min,sample volume was 3.5BV ,and 80% ethanol was used as eluting agent and its dosage was 10BV. ConclusionThe type of AB-8 showed comprehensive property in the condition and the yield of the total flavones achieved nearly 90%.
Key words:Herb Cirsii japonici; Total flavone ; Macroporous absorption resin
大蓟为菊科植物蓟 Cirsium japonicum Fisch. ex DC.的干燥地上部分或根,具有凉血止血、祛淤消痈的功能[1]。大蓟中含有多种化学成分[2]:黄酮、黄酮苷、生物碱、挥发油和谷甾醇等,其中黄酮类化合物柳穿鱼叶苷为主要止血活性成分[3]。