Determination of the Contents of Emodin and Chrysophanol in Fule Syrup by HPLC
LUO Yi,HUANG Zhuoqian
Guangxi Institute for Food and Drug Control, Nanning, 530001, China
Abstract:ObjectiveTo determine the contents of emodin and chrysophanol in Fule Syrup by HPLC.MethodsThe Kromasil C18 Column(250 mm×4.6 mm,5 μm)was used,the mobile phase was methanol-0.1% phosphoric acid(85∶15),the detective wavelength was at 254 nm,the flow rate was 1.0 ml·min-1.ResultsThe linear ranges of emodin and chrysophanol were at 0.021~0.53 μg and 0.019~0. 35 μg respectively,the average recoveries(n=9) were 100.9% and 101.1% with RSD 2.4% and 1.6% respectively.ConclusionThis method is sensitive and accurate.