Determination of Glibenclamide in XiaoTangLing Capsules by HPLC
CUI Zhe, GUO Jianpeng
1.The Hospital of Yanbian University,Yanji,Jilin 133000,China;
2. Pharmacy College of Yanbian University, Yanji,Jilin 133000,China
AbstractObjectiveTo research the methods of determination of Glibenclamide content in XiaoTangLing capsules by HPLC. MethodsHP ODS-Hypersil chromatographic column (4.6 mm×200 mm,5 μm ) was used to determine the content of Glibenclamide under the temperature of 20℃. Mobile phase: The ratio of methanol and 0.005mol/L phosphoric acid dihydroamine was 70:30 under the pH3.5. Flow rate: 0.8 ml / min. The wavelength of detection:300 nm. ResultsIn the range from 0.141μg to 2.25μg, Glibenclamide appeared fine linear correlation(r=0.999 9, n=5). The average recovery was 99.18 %,RSD= 1.72%(n=3). ConclusionHPLC method has some advantages, such as convenient, swift, sensitive, so HPLC can be used as a suitable method for determination of XiaoTangLing capsule.