Abstract:ObjectiveTo establish a quality standard for Liulingjiedu pill.Methods Calculus bovis in this medicine was identified by TLC. Cinobufagin and Resibufogenin in Linlingjiedu pill were determined by HPLC, using Zorbax C18 column and CH3CN∶KH2PO4:35∶65(adjust pH3.2) as the mobile phase. The detective wavelength was 296 nm.ResultsThe TLC spots developed were quite clear. Cinobufagin and resibufogenin showed a good linear relationship at a range of 0.3116 ~ 2.492 8 μg and 0.309 6 ~2.476 8 μg.The average recovery of Cinobufagin was 99.18 % and RSD was 1.55 %. The average recovery of Resibufogenin was 103.30% and RSD was 1.73 %.ConclusionThe method can be used for the quality control of Liulingjiedu pill.
Key wordsiulingjiedu pill; Cinobufagin; Resibufogenin; Quality standard