Optimization of Extracting Process for Bushenyijing Granules by Orthogonal Test
Abstract:ObjectiveTo optimize the extracting process for Bushenyijing Granules. MethodsWith content of icariin as the inspection indicators, percolation method and orthogonal test were designed for optimization of extracting process for Bushenyijing Granules. Orthogonal test was used to investigate the influence of four extracting factors (concentration of alcohol (A), alcohol volume (B), extracting time (C) and times of extracting (D)) on the extracting process.ResultsThe optimum percolation method was that the medicinal material was percolated at 1 to 3 ml/min after soaked for 48 hours in 70% alcohol. The optimum extracting process was A2D1C3B2, i.e. the 50% alcohol volume being 8 times of the weight of medicinal material, extraction for 1 times and 3 hours each time.ConclusionOptimization of extracting process for Bushenyijing Granules by orthogonal test is a simple, quick and accurate method.
Key words:Bushenyijing Granules; Icariin; Percolation method; Orthogonal test