Abstract:ObjectiveTo optimize the extraction procedure for trypsin inhibitor from Houttuynia cordata Thunb..MethodsThe inhibition rate of trypsin activity was used as screening parameter, we employed the orthogonal design method to test the effects of four factors, including temperature of extraction solution (A), volume of extraction solution (B), pH value of extraction solution(C) and time of extraction (D), on the extraction of trypsin inhibitor.ResultsFactor B performed the most significant effect on the extraction, and factors A and C came the next and factor D the last. ConclusionThe optimum extraction technology is A1B2C3D2 when the inhibition ratio of trypsin activity is considered as preferential parameter.
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